Identification of SARS-COV spike protein-derived and HLA-A2-restricted human CTL epitopes by using a new muramyl dipeptidederivative adjuvant.
Identifieur interne : 001847 ( Main/Exploration ); précédent : 001846; suivant : 001848Identification of SARS-COV spike protein-derived and HLA-A2-restricted human CTL epitopes by using a new muramyl dipeptidederivative adjuvant.
Auteurs : Y-Z Chen [Japon] ; G. Liu ; S. Senju ; Q. Wang ; A. Irie ; M. Haruta ; M. Matsui ; F. Yasui ; M. Kohara ; Y. NishimuraSource :
- International journal of immunopathology and pharmacology [ 0394-6320 ]
Descripteurs français
- KwdFr :
- Acétylmuramyl alanyl isoglutamine (analogues et dérivés), Adjuvants immunologiques (pharmacologie), Animaux, Antigène HLA-A2 (immunologie), Antigènes viraux (immunologie), Déterminants antigéniques des lymphocytes T, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Immunisation, Lymphocytes T cytotoxiques (immunologie), Protéines de l'enveloppe virale (immunologie), Présentation d'antigène, Souris, Souris de lignée C57BL, Souris transgéniques, Vaccins antiviraux (immunologie).
- MESH :
- analogues et dérivés : Acétylmuramyl alanyl isoglutamine.
- immunologie : Antigène HLA-A2, Antigènes viraux, Glycoprotéines membranaires, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Vaccins antiviraux.
- pharmacologie : Adjuvants immunologiques.
- Animaux, Déterminants antigéniques des lymphocytes T, Glycoprotéine de spicule des coronavirus, Humains, Immunisation, Présentation d'antigène, Souris, Souris de lignée C57BL, Souris transgéniques.
English descriptors
- KwdEn :
- Acetylmuramyl-Alanyl-Isoglutamine (analogs & derivatives), Adjuvants, Immunologic (pharmacology), Animals, Antigen Presentation, Antigens, Viral (immunology), Epitopes, T-Lymphocyte, HLA-A2 Antigen (immunology), Humans, Immunization, Membrane Glycoproteins (immunology), Mice, Mice, Inbred C57BL, Mice, Transgenic, Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , analogs & derivatives : Acetylmuramyl-Alanyl-Isoglutamine.
- chemical , immunology : Antigens, Viral, HLA-A2 Antigen, Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , pharmacology : Adjuvants, Immunologic.
- immunology : T-Lymphocytes, Cytotoxic.
- Animals, Antigen Presentation, Epitopes, T-Lymphocyte, Humans, Immunization, Mice, Mice, Inbred C57BL, Mice, Transgenic, Spike Glycoprotein, Coronavirus.
Abstract
Severe acute respiratory syndrome (SARS) spread during the winter of 2003, and attempts have been made to develop vaccines against SARS corona virus (SARS-CoV). The present study provides a strategy to rapidly identify SARS-CoV-derived antigenic peptides recognized by HLA-A2-restricted cytotoxic T lymphocytes (CTLs). Forty-three candidate peptides having HLA-A2-binding motifs were selected in silico and HLA-A2/Db chimeric MHC class I-transgenic mice were immunized with these peptides and a new derivative of muramyl dipeptide that can induce upregulation of HLA-DR, CD80, CD86, and CD40 in human CD14+ antigen presenting cells, was administered as an adjuvant. Six HLAA2-restricted mouse CTL epitopes were identified, including two new epitopes which have never been reported before. One of the novel peptides was naturally processed and successfully induced HLAA2-restricted specific CTLs in both HLA transgenic mice and healthy donors. The method was useful, convenient and efficient for rapid identification of CTL epitopes derived from SARS-CoV proteins and will be possibly applicable for other pathogens to develop a peptide-based vaccine.
DOI: 10.1177/039463201002300115
PubMed: 20378004
Affiliations:
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Le document en format XML
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Senju</name></author><author><name sortKey="Wang, Q" sort="Wang, Q" uniqKey="Wang Q" first="Q" last="Wang">Q. Wang</name></author><author><name sortKey="Irie, A" sort="Irie, A" uniqKey="Irie A" first="A" last="Irie">A. Irie</name></author><author><name sortKey="Haruta, M" sort="Haruta, M" uniqKey="Haruta M" first="M" last="Haruta">M. Haruta</name></author><author><name sortKey="Matsui, M" sort="Matsui, M" uniqKey="Matsui M" first="M" last="Matsui">M. Matsui</name></author><author><name sortKey="Yasui, F" sort="Yasui, F" uniqKey="Yasui F" first="F" last="Yasui">F. Yasui</name></author><author><name sortKey="Kohara, M" sort="Kohara, M" uniqKey="Kohara M" first="M" last="Kohara">M. Kohara</name></author><author><name sortKey="Nishimura, Y" sort="Nishimura, Y" uniqKey="Nishimura Y" first="Y" last="Nishimura">Y. 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Liu</name></author><author><name sortKey="Senju, S" sort="Senju, S" uniqKey="Senju S" first="S" last="Senju">S. Senju</name></author><author><name sortKey="Wang, Q" sort="Wang, Q" uniqKey="Wang Q" first="Q" last="Wang">Q. Wang</name></author><author><name sortKey="Irie, A" sort="Irie, A" uniqKey="Irie A" first="A" last="Irie">A. Irie</name></author><author><name sortKey="Haruta, M" sort="Haruta, M" uniqKey="Haruta M" first="M" last="Haruta">M. Haruta</name></author><author><name sortKey="Matsui, M" sort="Matsui, M" uniqKey="Matsui M" first="M" last="Matsui">M. Matsui</name></author><author><name sortKey="Yasui, F" sort="Yasui, F" uniqKey="Yasui F" first="F" last="Yasui">F. Yasui</name></author><author><name sortKey="Kohara, M" sort="Kohara, M" uniqKey="Kohara M" first="M" last="Kohara">M. Kohara</name></author><author><name sortKey="Nishimura, Y" sort="Nishimura, Y" uniqKey="Nishimura Y" first="Y" last="Nishimura">Y. Nishimura</name></author></analytic><series><title level="j">International journal of immunopathology and pharmacology</title><idno type="ISSN">0394-6320</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetylmuramyl-Alanyl-Isoglutamine (analogs & derivatives)</term><term>Adjuvants, Immunologic (pharmacology)</term><term>Animals</term><term>Antigen Presentation</term><term>Antigens, Viral (immunology)</term><term>Epitopes, T-Lymphocyte</term><term>HLA-A2 Antigen (immunology)</term><term>Humans</term><term>Immunization</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acétylmuramyl alanyl isoglutamine (analogues et dérivés)</term><term>Adjuvants immunologiques (pharmacologie)</term><term>Animaux</term><term>Antigène HLA-A2 (immunologie)</term><term>Antigènes viraux (immunologie)</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Immunisation</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Présentation d'antigène</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term><term>Vaccins antiviraux (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Acetylmuramyl-Alanyl-Isoglutamine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>HLA-A2 Antigen</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acétylmuramyl alanyl isoglutamine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigène HLA-A2</term><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antigen Presentation</term><term>Epitopes, T-Lymphocyte</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunisation</term><term>Présentation d'antigène</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) spread during the winter of 2003, and attempts have been made to develop vaccines against SARS corona virus (SARS-CoV). The present study provides a strategy to rapidly identify SARS-CoV-derived antigenic peptides recognized by HLA-A2-restricted cytotoxic T lymphocytes (CTLs). Forty-three candidate peptides having HLA-A2-binding motifs were selected in silico and HLA-A2/Db chimeric MHC class I-transgenic mice were immunized with these peptides and a new derivative of muramyl dipeptide that can induce upregulation of HLA-DR, CD80, CD86, and CD40 in human CD14+ antigen presenting cells, was administered as an adjuvant. Six HLAA2-restricted mouse CTL epitopes were identified, including two new epitopes which have never been reported before. One of the novel peptides was naturally processed and successfully induced HLAA2-restricted specific CTLs in both HLA transgenic mice and healthy donors. The method was useful, convenient and efficient for rapid identification of CTL epitopes derived from SARS-CoV proteins and will be possibly applicable for other pathogens to develop a peptide-based vaccine.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Haruta, M" sort="Haruta, M" uniqKey="Haruta M" first="M" last="Haruta">M. Haruta</name><name sortKey="Irie, A" sort="Irie, A" uniqKey="Irie A" first="A" last="Irie">A. Irie</name><name sortKey="Kohara, M" sort="Kohara, M" uniqKey="Kohara M" first="M" last="Kohara">M. Kohara</name><name sortKey="Liu, G" sort="Liu, G" uniqKey="Liu G" first="G" last="Liu">G. Liu</name><name sortKey="Matsui, M" sort="Matsui, M" uniqKey="Matsui M" first="M" last="Matsui">M. Matsui</name><name sortKey="Nishimura, Y" sort="Nishimura, Y" uniqKey="Nishimura Y" first="Y" last="Nishimura">Y. Nishimura</name><name sortKey="Senju, S" sort="Senju, S" uniqKey="Senju S" first="S" last="Senju">S. Senju</name><name sortKey="Wang, Q" sort="Wang, Q" uniqKey="Wang Q" first="Q" last="Wang">Q. Wang</name><name sortKey="Yasui, F" sort="Yasui, F" uniqKey="Yasui F" first="F" last="Yasui">F. Yasui</name></noCountry><country name="Japon"><noRegion><name sortKey="Chen, Y Z" sort="Chen, Y Z" uniqKey="Chen Y" first="Y-Z" last="Chen">Y-Z Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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